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eBook details

• Title: Limitations of Steroid Determination by Direct Immunoassay (Technical Briefs)
• Author : Clinical Chemistry
• Release Date : January 01, 2002
• Genre: Chemistry,Books,Science & Nature,
• Pages : * pages
• Size : 166 KB

Description

Rapid steroid hormone immunoassays often agree poorly, especially at normal and low concentrations (1-4). These problems result from low assay specificity, inadequate standardization, and poor optimization of the methods over the large range of concentrations seen clinically (5-7). These systems are often unsuitable for clinical applications that require a low detection limit, such as the following: (a) estradiol measurements in men [110 pmol/L; (30 pg/mL)] or children [from 18 pmol/L to 165 pmol/L (5 pg/mL to 45 pg/mL)] (8) and evaluation of down-regulation by gonadoliberin analogs before in vitro fertilization and embryo transfer (1VF-ET) programs; (b) progesterone determinations during ovarian stimulation, with values 3.2 nmol/L (1 ng/mL) on the day of human chorionic gonadotropin administration predictive for pregnancy in 1VF-ET (9,10); (c) testosterone assays for children [from 0.35 nmol/L to 5 nmol/L (0.1 ng/mL to 1.5 ng/mL)] and women [2.4 nmol/L (0.7 ng/mL)] (11,12). Furthermore, limits of detection determined with the zero calibrator are generally far below the lowest concentration that can be reliably quantified in human serum [functional sensitivity (13,14) or limit of quantitation (LOQ) (15)]. In this study, we analyzed and compared detection limits and functional sensitivities for nine estradiol ([E.sub.2]) and eight progesterone (P) immunoassays.

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